![]() Sea buckthorn ( Elaeagnus rhamnoides (L.) A. Moreover, it is very important from an economic point of view to know that there is a possibility of obtaining phenolic compounds not only from the berries or leaves, but also from twigs, which constitute a production waste. Our present results have demonstrated that extracts from different parts of SBT, especially berries and twigs, in comparison to well-known berries (aronia and grape), may also be viewed as a good source of active substances – antioxidants for pharmacological or cosmetic applications. Both extracts also showed anticoagulant properties. SBT twig and leaf extracts were observed to exhibit an antioxidant activity against two strong biological oxidants: hydrogen peroxide (H 2O 2) and H 2O 2/Fe (the donor of hydroxyl radicals), which induced human plasma lipid peroxidation and protein carbonylation. Additionally, we analysed the potential mechanism of modulation of hemostatic properties of human plasma (using selected coagulation times). the level of carbonyl groups in proteins. We determined the effect of plant extracts on the oxidative stress using selected markers of this process, i.e. Moreover, the aim of present experiments was to compare the biological activity of SBT leaf extract and SBT twig extract with selected berry extracts (a rich source of phenolic compounds): SBT berry extract (flavonoids being the dominant components), a commercial extract from the berries of Aronia melanocarpa (Aronox ®), and a grape seed extract. The present study was carried out in order to investigate antioxidant and anticoagulant properties of sea buckthorn twig and leaf extracts (0.5–50 μg/mL) by using various in vitro models. However, not only sea buckthorn berries, but also leaves of this plant (both fresh and dried) contain a lot of nutrients and bioactive compounds, including phenolic compounds. Different bioactive compounds in SBT berries are of special interest to various researchers. Nelson, SBT) is a valuable plant because of its medical and therapeutic potential. sphaerica on mango in Egypt, which might severely affect the local production of mango.Sea buckthorn ( Elaeagnus rhamnoides (L.) A. To the best of our knowledge, this is the first report of twig dieback and leaf spot caused by N. The same fungus was re-isolated and re-identified. After 40/15 days of incubation at 24 ☌ in moist chambers, only inoculated plants/leaves had similar symptoms to those observed on affected trees. ![]() Control plants/leaves were inoculated with sterile PDA plugs. Meantime, 30 detached, surface-sterilized leaves were wounded and inoculated with a 0.5 cm-mycelial plug. To fulfill Koch’s postulates, 10-month-old mango seedlings were inoculated with a 0.5 cm-mycelial plug at the growing tip region of the stem. Sequences were deposited in GenBank (ITS, MW704467 TEF1-α, ON428976) and proved to 99.6 and 91.4% match in BLAST searches with N. Identity of EGYARC1 was confirmed by sequencing portions of rDNA-ITS region and TEF1-α gene (White et al. sphaerica EGYARC1 produced single celled, black, spherical to sub-spherical conidia, 10 to 14 × 18 to 20 μm, borne on a hyaline vesicle at the tip of conidiophore. The isolated fungus (70% frequency) was identified as Nigrospora sphaerica (Sacc.) Mason (Wright et al. Twenty leaves and wood portions from 20 diseased trees were surface sterilized in 1% sodium hypochlorite for 2 min, placed onto potato dextrose agar (PDA) and incubated in dark at 24 ☌. Dieback was characterized by the discoloration of wood and downward drying out of the twigs. Spots appeared yellow to brown, with dry grey necrotic centers, irregularly distributed on leaves. Three different locations were inspected (20 farms/location), with an incidence in the affected sites of 100%. Ewais trees cultivated in El Sharkia governorate, Egypt. During 2021, twig dieback and leaf spots were observed on mango cv.
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